The Production of Streptomyces W-5B Extract for Antibiofilm against Methicillin-resistant Staphylococcus aureus

The ability of Methicillin-resistant Staphylococcus aureus (MRSA) to form biofilms is one the triggering factors for emergence MRSA resistance antibiotics. Streptomyces W-5B has shown potency as an antibacterial producer against MRSA. However, production microbial bioactive compounds strongly affected by source nutrients in fermentation medium. Therefore, objective this study was determine optimal sources carbon and nitrogen with antibiofilm activities. research method included cultivating W-5B, extract production, variation (glucose, sucrose, starch) (casein, peptone, urea) Antibiofilm activities were measured based on inhibition biofilm formation degradation tests using microtiter plate a crystal violet stain. results showed that highest 68.206 ± 1.750% after 12 days incubation medium containing sucrose urea. Meanwhile, 73.023 1.972% nine starch These findings indicated produce